Effects of beta-glucan in a mice model of vaginitis by candida albicans
نویسندگان
چکیده
Methods Fifty four Balb/C mice of 7 to 10 weeks old under influence of estrogen were inoculated with 5x10 stationaryphase blastoconidia of C. albicans, intravaginally. The mice were divided in three groups, treated with glucan vaginally (5mg/mL) and intraperitoneally (1mg/mL), and the control group that received saline, intraperitoneally. Vaginal lavage was obtained on days 2, 5, 8 e 10 after inoculation with C. albicans for the count of CFU by pour-plate method. Moreover, in the days 6, 9 and 11 after inoculation, three mice from each group were sacrificed and the vaginas were removed for histophatological analysis. The slides were stained by hematoxylineosin to evaluate the infiltrate of neutrophils and periodic acid-Schiff (PAS) for analyze of fungal burden.
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